Colo205 In Vitro BrdU Assay (Cell Proliferation)

CAT#: ITS-1022-YF539
Target Cell Organism: Human
Target Cell Alternative Name: COLO 205
Target Cell Name: Colo205
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Colo205-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Colo205
Target Cell Organism
Human
Target Cell Background
The COLO 205 cell line is made up of epithelial cells isolated in 1975 from ascitic fluid derived from a White, 70-year-old, man with colon cancer. The cells can be used for cancer and toxicology research.
Target Cell Alternative Name
COLO 205
Related Diseases
Colon Cancer
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Short Description
Colo205-cell based In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent signal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Proliferation Assay
Assay MOA
Cell-based Assays Based on DNA Synthesis
For Research Use Only | Not For Clinical Use
Online Inquiry