Colo205 In Vitro RNA Expression Analysis Assay (SAGE)

This assay is to provide Colo205-based In Vitro RNA Expression Analysis Assay (SAGE) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Colo205

Human

The COLO 205 cell line is made up of epithelial cells isolated in 1975 from ascitic fluid derived from a White, 70-year-old, man with colon cancer. The cells can be used for cancer and toxicology research.

COLO 205

Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (SAGE)

Colo205-cell based In Vitro RNA Expression Analysis Assay (SAGE)

Serial analysis of gene expression (SAGE) is another molecular biological technique used to analyze gene expression. In this technique, mRNA extracted from the sample is reverse transcribed into cDNA and the resulting cDNA is then digested by restriction enzymes to produce tags. These tags are amplified by PCR and subjected to sequencing. Several modifications to SAGE such as Long SAGE, RL-SAGE and Super SAGE have been introduced to improve the SAGE technique.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.