Cow IL1A ELISA Kit, Lot 21AO-315 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P226)

IL-1 alpha Microplate (Item A): 96 well PCR plate coated with anti-Bovine IL-1 alpha.
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
Standards (Item C): 2 vials of recombinant Bovine IL-1 alpha.
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer.
Detection Affinity Reagent for IL-1 alpha (Item F): 2 vials of a 4x concentrated solution of anti-Bovine IL-1 alpha affinity reagent.
Quantitative ELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock.
Primer Solution (Item I): 1.7 ml vial.
PCR Master Mix (Item J): 1.2 ml vial.
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer.
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

1.6 pg/mL

25 μL

Pre-coated

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

For Research Use only

Interleukin-1-alpha (IL-1-alpha)

IL-1A; IL1; IL1-ALPHA; IL1F1; Il-1a; IL-1 alpha; IL-1alpha; L1A; interleukin 1 alpha; IL1A; Il1a

NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.