Cow Selectin E + CD62e (SELE) ELISA Kit, Lot 21AO-923 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P834)

Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30 x concentrate)
Instruction manual

1.Microplate reader with 450 ± 10nm filter.
2.Precision single or multi-channel pipettes and disposable tips.
3.Eppendorf Tubes for diluting samples.
4.Deionized or distilled water.
5.Absorbent paper for blotting the microtiter plate.
6.Container for Wash Solution
7.0.01Mol/L (or 1x) Phosphate Buffered Saline(PBS), pH 7.0-7.2.

0.21 ng/mL

100 μL

1 - 4.5 h

Pre-coated

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated.

4 °C,-20 °C

The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received.

12 months

The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received.

For Research Use only

E-selectin

SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2; E-selectin; Elam; selectin E; selectin; endothelial cell; SELE; Sele

Alternative name: CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E

281484

P98107

Thromboxane A2 Receptor Signaling

1.Prepare all reagents, samples and standards,
2.Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3.Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4.Aspirate and wash 3 times,
5.Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6.Aspirate and wash 5 times,
7.Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8.Add 50μL Stop Solution. Read at 450nm immediately.