Custom Human EGFR Sandwich ELISA Kit-qPCR

CAT#: TM-HMM077
Product Type: Sandwich ELISA kit
Target: BCMA
Reactivity: Human
Applications: BCMA detection
Description
This product is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR.
Features
High sensitivity qPCR readout for the quantitative measurement of human BCMA.
Applications
BCMA detection
Target
BCMA
Reactivity
Human
Detection Method
qPCR
Method Type
Quantitative
Sample Type
Cell culture, supernatants, plasma, serum
Size
96-well microplate
Receptor Family
TNF receptor superfamily
Qualified With
Certificate of quality
Components
BCMA microplate (Item A); Wash buffer; Standards; Assay diluent A; Assay diluent B; Detection affinity reagent for BCMA; Detection reagent; Primer solution; PCR master mix; PCR preparation buffer; Final wash buffer
Sensitivity
4.8 pg/mL
Plate
1, 2, or 5
Reagent Preparation
Real-time PCR instrument, Bio-Rad recommended; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; 100 mL and 1 liter graduated cylinders; Absorbent paper; Distilled or deionized water; Log-log graph paper or computer and software for data analysis; Tubes to prepare standard or sample dilutions; Heating block or water bath capable of 80°C
Assay Procedure
Prepare all reagents, samples and standards as instructed; Add 25µL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C; Add 25 µL detection affinity reagent to each well. Incubate 1 hour at room temperature; Add 25µL of Detection Reagent to each well. Incubate 1 hour; Add 15µL Primer solution 10µL of PCR master mix to each well; Run real-time PCR
Target/Description
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.
Accession Number
Q02223
Storage
Stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
TNFRSF17; B cell maturation antigen; BCMA; CD269 antigen; B-cell maturation factor; B-cell maturation protein; BCM; CD269
Synonyms
TNFRSF17; B cell maturation antigen; BCMA; CD269 antigen; B-cell maturation factor; B-cell maturation protein; BCM; CD269
Background
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.
Gene ID
608
For Research Use Only | Not For Clinical Use
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