Custom Human Phospho-ErbB3 (Tyr1262) and Total ErbB3 ELISA Kit

This ELISA kit recognizes human ErbB3 phosphorylated at site tyrosine-1262 as well as total ErbB3.

Rapidly measure phosphorylated protein in adherent cell lines; Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose); No sample lysis is needed; Compatible with a standard ELISA plate reader.

ERBB3/HER3 detection

ERBB3/HER3

Human

Colorimetric

Semi-quantitative

Adherent cell culture

96-well plates

Epidermal growth factor receptor (EGFR/ERBB) family

Certificate of quality

Uncoated 96-well strip microplate; Wash buffers; Fixing solution; Quenching buffer; Blocking buffer; Anti-phospho antibody; Anti-pan antibody; HRP-conjugated secondary antibody; TMB one-step substrateStop solution

1, 2, or 5

Distilled or deionized water; 100 mL and 1 liter graduated cylinders; Tubes to prepare sample dilutions; Protease and Phosphatase inhibitors; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; Benchtop rocker or shaker; Microplate reader capable of measuring absorbance at 450 nm.

Seed 10,000-30,000 cells into each well and incubate overnight; Apply various treatment, inhibitors or activators according to manufacturer's instructions; Add 100 µL of Fixing Solution into each well and incubate for 20 min at RT with shaking; Add 200 µL of prepared 1X Quenching Buffer and incubate 20 min at RT; Add 200 µL of Blocking Solution and incubate for 1 h at 37°C; Add 50 µL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT; Add 50 µL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT; Add 100 µL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT; Add 50 µL of Stop Solution to each well; Read at 450 nm immediately.

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.

P21860

-20°C

For research use only. Intended for use by laboratory professionals.

ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3

ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.

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