Custom Rat EGFR Sandwich ELISA Kit-qPCR

CAT#: TM-HMM057
Product Type: Sandwich ELISA kit
Target: EGFR
Reactivity: Rat
Applications: EGFR detection
Description
This product is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR.
Features
High sensitivity qPCR readout for the quantitative measurement of rat EGFR in serum, plasma, and cell culture supernatants.
Applications
EGFR detection
Target
EGFR
Reactivity
Rat
Detection Method
qPCR
Method Type
Quantitative
Sample Type
Cell culture supernatants, plasma, serum
Size
96-well microplate
Receptor Family
Epidermal growth factor family (EGF family)
Qualified With
Certificate of quality
Components
EGFR microplate; Wash buffer; Standards; Assay diluent A; Assay diluent B; Detection affinity reagent for EGFR; Detection reagent; Primer solution; PCR master mix; PCR preparation buffer; Final wash buffer
Sensitivity
Typically 10x lower than traditional ELISA
Plate
1, 2, or 5
Reagent Preparation
Real-time PCR instrument, Bio-Rad recommended; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; 100 mL and 1 liter graduated cylinders; Absorbent paper; Distilled or deionized water; Log-log graph paper or computer and software for data analysis; Tubes to prepare standard or sample dilutions; Heating block or water bath capable of 80°C
Assay Procedure
Prepare all reagents, samples and standards as instructed; Add 25µL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C; Add 25 µL detection affinity reagent to each well; Incubate 1 hour at room temperature; Add 25µL of Detection Reagent to each well; Incubate 1 hour; Add 15µL Primer solution 10µL of PCR master mix to each well; Run real-time PCR
Target/Description
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
Accession Number
G3V6K6
Storage
Stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Synonyms
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Background
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
For Research Use Only | Not For Clinical Use
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