CypMaster™ Multi-Isoform CYPs Metabolite Kits

Generates diverse Phase I and Phase II drug metabolites using a broad-spectrum panel of recombinant biocatalytic enzymes.

CypMaster™

ADME

Metabolism

CypMaster™ Multi-Isoform CYPs Metabolite Kits provide a scalable, biocatalytic solution for synthesizing difficult-to-reach drug metabolites. By utilizing a diverse array of recombinant CYP450 isoforms and Phase II enzymes, this system enables the rapid production of milligram-to-gram quantities of metabolites for structural elucidation, toxicity testing, and use as analytical standards.

Expanded Biocatalytic Diversity: Features a comprehensive panel of recombinant enzymes including human CYPs, microbial isoforms, and Phase II transferases for broad metabolic coverage.
Scalable Metabolite Production: Engineered for seamless transition from analytical-scale screening to preparative-scale synthesis of milligram quantities for definitive structural characterization.
Superior Chemical Versatility: Capable of performing complex oxidations, hydroxylations, and conjugations on diverse chemical scaffolds where traditional synthetic chemistry is challenging.
Simplified Purification Workflow: Utilizes high-purity recombinant catalysts that minimize biological matrix interference, facilitating easier downstream isolation and LC-MS/MS or NMR analysis.

Generates authentic metabolite standards and derivatives for structural identification and biological activity assessment during lead optimization and safety testing.

Lead optimization, metabolite safety testing (MIST), and production of reference standards for clinical ADME and pharmacokinetic studies.

Synthesizing Phase I/II metabolites, structural elucidation via NMR, bioactivity screening of drug derivatives, ADME profiling studies, and providing reference standards for quantitative LC-MS/MS.

Internal performance validation using reference standards under defined assay conditions.

Diverse drug-metabolizing enzymes

Reaction substrates, small-molecule compounds, drug candidates, and chemical probes.

Quantitative

Pharmacokinetics (ADME), metabolite safety testing (MIST), drug discovery, medicinal chemistry, etc.

Synthesis of purified metabolite compounds and structural characterization data including mass spectrometry and NMR spectra.

Human

Quantitative

Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.

Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.

12 months under recommended storage conditions to ensure optimal performance.

For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.

For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.

Shipped on ice under temperature-controlled conditions.

Drug metabolism often produces reactive or pharmacologically active derivatives that must be characterized for regulatory approval. Biocatalytic systems offer a sustainable and efficient alternative to complex chemical synthesis for producing these specific standards.

These kits simulate hepatic Phase I oxidation and Phase II conjugation pathways. They leverage recombinant enzymes to mimic systemic clearance mechanisms, facilitating the enzymatic conversion of parent drugs into polar, excretable metabolites.