CytoFinal™ Chicken Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB85
Product Type: ELISA Kit
Species: Chicken
Target: Chicken cytoplasmic Aspartate Aminotransferase 1 (GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in chicken tissues, cells, and biological fluids.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Chicken AST (GOT1) ELISA Kit provides a reliable, high-sensitivity in vitro solution for detecting cytoplasmic aspartate aminotransferase in chicken-derived samples. The sandwich ELISA format enables precise quantification in tissue homogenates, cell lysates, and other biological fluids, supporting hepatology, immunology, and cancer research studies.
Features
High-sensitivity detection of chicken GOT1 with a quantification range from 0.312 to 20 ng/mL, ensuring accurate low-level measurement.
Colorimetric sandwich ELISA design enables robust quantification through HRP-mediated TMB substrate conversion for reliable data.
Pre-coated 96-well plate format simplifies workflow, reduces variability, and supports high-throughput sample analysis in research laboratories.
Lyophilized standards and reagents with stable storage provide consistent assay performance and minimize activity loss over extended shelf life.
Process Relevance
Monitoring chicken cytoplasmic AST activity to support liver function assessment and metabolic profiling in research studies.
Application Stage
Drug discovery, nutritional studies, hepatology research, and immunological assays.
Applications
Suitable for quantitative ELISA analysis in chicken tissue homogenates, cell lysates, serum, plasma, and other ADME profiling studies.
Target
Chicken cytoplasmic Aspartate Aminotransferase 1 (GOT1)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzyme regulation, hepatology, cancer biology, metabolic pathway analysis, pharmacokinetics (ADME), avian immunology, translational liver research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of cytoplasmic AST (GOT1) enzyme concentration in biological samples.
Assay Range
0.312 ng/ml - 20 ng/ml
LOD
< 0.14 ng/ml
Species
Chicken
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aspartate aminotransferase (AST/GOT1) is a critical enzyme in amino acid metabolism, catalyzing the reversible conversion of aspartate and α-ketoglutarate. Its cytoplasmic form reflects cellular metabolic activity and liver health in avian models, providing insight into hepatocellular injury, immune responses, and metabolic regulation.
Pathways
Cytoplasmic AST (GOT1) participates in the malate-aspartate shuttle and amino acid metabolism, linking nitrogen balance to energy production. Dysregulation may affect hepatic transamination, nitrogen disposal, and downstream Krebs cycle intermediates, impacting cellular metabolism and stress responses.
For Research Use Only | Not For Clinical Use
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