CytoFinal™ High-Sensitivity Rat Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB92
Product Type: ELISA Kit
Species: Rat
Target: Rat cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Precisely quantifies cytoplasmic AST (GOT1) levels in rat biological samples with enhanced sensitivity and reliability.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High-Sensitivity Rat AST (GOT1) ELISA Kit provides an advanced in vitro method for detecting cytoplasmic AST in rat serum, plasma, tissues, and cell lysates. Optimized for low-abundance detection, this kit enables accurate assessment of metabolic and hepatocellular function in preclinical research models.
Features
Employs high-sensitivity sandwich ELISA technology to detect minute cytoplasmic AST levels in rat serum, plasma, tissues, and cell lysates.
Offers excellent reproducibility and precision with standardized reagents and lyophilized controls for consistent assay performance.
Compatible with multiple rat sample types, supporting diverse experimental designs and preclinical hepatology studies.
Provides a wide dynamic range and rapid detection, facilitating reliable low-level AST quantification under variable conditions.
Process Relevance
Supports precise monitoring of cytoplasmic AST to assess hepatocellular integrity and metabolic status in rat preclinical studies.
Application Stage
Applicable in preclinical research, hepatology studies, drug safety evaluation, and laboratory-based quality control.
Applications
Sensitive detection of cytoplasmic AST in rat serum, plasma, tissues, and cell lysates for hepatotoxicity studies, biomarker discovery, ADME profiling studies, and metabolic pathway analysis.
Target
Rat cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolism, enzymology, biomarker discovery, preclinical pharmacology, pharmacokinetics (ADME), translational research, toxicology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST activity in rat biological samples.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 mediates reversible transamination between aspartate and α-ketoglutarate, essential for amino acid metabolism and TCA cycle integration. Cytoplasmic AST levels provide critical insights into hepatocellular function and metabolic health in preclinical models.
Pathways
AST is a key component of the malate-aspartate shuttle, regulating cytoplasmic-mitochondrial redox balance and facilitating energy production. Its activity influences TCA cycle flux and amino acid homeostasis.
For Research Use Only | Not For Clinical Use
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