CytoFinal™ Human Alanine Aminotransferase 2 / ALT (GPT2) ELISA Kit

CAT#: ITS-0326-WB82
Product Type: ELISA Kit
Species: Human
Target: Detects Human Alanine Aminotransferase 2 (GPT2) protein
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures Human ALT2 (GPT2) levels in tissues, cells, and biological fluids using ELISA.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Alanine Aminotransferase 2 / ALT (GPT2) ELISA Kit enables sensitive and accurate in vitro quantification of ALT2 concentrations in tissue homogenates, cell lysates, and other biological fluids, providing reliable data for metabolic and disease-related research.
Features
Employs high-sensitivity sandwich ELISA technology, detecting ALT2 concentrations as low as 0.78 ng/mL in diverse biological samples.
Pre-coated 96-well plate format ensures reproducibility, minimizing user variability and supporting high-throughput experimental workflows.
Colorimetric detection with TMB substrate delivers proportional signal intensity, facilitating precise quantification of ALT2 enzyme levels.
Lyophilized standards and reagents provide extended shelf life and stability under proper storage conditions.
Process Relevance
Supports monitoring ALT2 enzymatic levels to assess liver function, metabolic activity, and cellular energy regulation.
Application Stage
Applicable in drug discovery, metabolic research, and in vitro disease modeling studies.
Applications
Enables ELISA-based quantification of ALT2 from tissue homogenates, cell lysates, and biological fluids for ADME profiling studies and disease research.
Target
Detects Human Alanine Aminotransferase 2 (GPT2) protein
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic disease research, liver function analysis, cellular energy metabolism, pharmacokinetics (ADME), drug toxicity assessment, enzymology studies, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement using colorimetric sandwich ELISA.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.78 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT2 (GPT2) is a mitochondrial enzyme catalyzing alanine-pyruvate conversion, crucial for amino acid metabolism and gluconeogenesis. Abnormal ALT2 levels correlate with liver dysfunction, metabolic disorders, and cellular energy dysregulation.
Pathways
ALT2 participates in amino acid catabolism and gluconeogenesis pathways, linking alanine metabolism to pyruvate production. Upstream regulators include cellular energy status, while downstream effects influence glucose homeostasis and mitochondrial function.
For Research Use Only | Not For Clinical Use
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