CytoFinal™ Human Lactate Dehydrogenase D (LDHD) ELISA Kit

CAT#: ITS-0326-WB117
Product Type: ELISA Kit
Species: Human
Target: Human D-lactate dehydrogenase (LDHD)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for detecting human D-lactate dehydrogenase (LDHD) in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Lactate Dehydrogenase D (LDHD) ELISA Kit enables sensitive and accurate quantification of LDHD protein in human-derived biological samples. Utilizing a high-performance sandwich ELISA with HRP-mediated colorimetric detection, this assay provides reliable measurement of LDHD levels in serum, plasma, tissue lysates, and cell extracts for metabolic and disease research.
Features
Highly specific sandwich ELISA using optimized antibody pairs enables precise detection of human LDHD with minimal interference from related metabolic enzymes.
Sensitive assay design with broad linear range supports accurate quantification of LDHD across multiple biological sample matrices.
HRP-TMB colorimetric detection produces stable absorbance signals compatible with standard microplate readers for efficient and reproducible laboratory analysis.
Complete kit configuration including precoated microplates, standards, buffers, and detection reagents simplifies experimental setup and improves assay consistency.
Process Relevance
Measures LDHD levels to evaluate D-lactate metabolism and mitochondrial metabolic regulation.
Application Stage
Metabolic research, clinical biomarker studies, drug discovery, and translational biomedical research.
Applications
Quantification of human LDHD in serum, plasma, tissue lysates, and cultured cells for metabolic pathway analysis, mitochondrial studies, disease biomarker research, ADME profiling studies, and experimental metabolism investigations.
Target
Human D-lactate dehydrogenase (LDHD)
Detection Method
Colorimetric
Sample Type
Serum, plasma and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic biology, mitochondrial metabolism, clinical biomarker discovery, toxicology, gastrointestinal metabolism research, pharmacokinetics (ADME), cellular metabolism, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHD protein concentration determined by ELISA absorbance.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
D-lactate dehydrogenase (LDHD) is a mitochondrial enzyme responsible for catalyzing the oxidation of D-lactate to pyruvate. Although less abundant than L-LDH isoforms, LDHD plays an important role in regulating D-lactate metabolism and maintaining metabolic balance. Alterations in D-lactate metabolism have been associated with mitochondrial dysfuncti
Pathways
LDHD participates in D-lactate metabolic pathways by catalyzing the conversion of D-lactate into pyruvate, linking D-lactate turnover to central carbon metabolism. The generated pyruvate can subsequently enter the tricarboxylic acid (TCA) cycle for energy production. Through this role, LDHD contributes to mitochondrial metabolic homeostasis and cellular energy regulation.
For Research Use Only | Not For Clinical Use
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