CytoFinal™ Mouse Lactate Dehydrogenase (LDH) ELISA Kit

CAT#: ITS-0326-WB107
Product Type: ELISA Kit
Species: Mouse
Target: Mouse Lactate Dehydrogenase (LDH)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for measuring mouse lactate dehydrogenase levels in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Mouse Lactate Dehydrogenase (LDH) ELISA Kit enables accurate in vitro quantification of LDH concentrations in mouse-derived biological samples. Based on a sandwich ELISA format with colorimetric detection, this assay supports sensitive measurement of LDH in serum, plasma, tissue homogenates, and related sample matrices for metabolic and cellular injury studies.
Features
High-affinity antibody-based sandwich ELISA ensures sensitive detection of mouse LDH with minimal cross-reactivity.
Broad dynamic range supports accurate quantification of LDH across diverse biological samples.
Colorimetric HRP-TMB detection enables reliable measurement using standard microplate readers at 450 nm.
Complete kit format including pre-coated plates, standards, buffers, and detection reagents for streamlined workflows.
Process Relevance
Quantifies LDH release to evaluate cellular damage, metabolic activity, and cytotoxicity in experimental models.
Application Stage
Drug discovery, disease modeling, metabolic studies, and preclinical biomarker research.
Applications
Measurement of LDH levels in mouse serum, plasma, and tissue homogenates to assess cell damage, metabolic activity, inflammation, ADME profiling, and treatment-induced cytotoxicity.
Target
Mouse Lactate Dehydrogenase (LDH)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cancer metabolism, immunology and inflammation, cardiovascular research, liver disease studies, pharmacokinetics (ADME), metabolic regulation, toxicology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDH concentration based on sandwich ELISA absorbance values.
Assay Range
7.8 ng/ml - 500 ng/ml
LOD
< 3.10 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase (LDH) is a cytoplasmic enzyme widely distributed in mammalian tissues and plays a central role in anaerobic energy metabolism. Elevated extracellular LDH levels commonly indicate cellular membrane damage, tissue injury, or metabolic stress. Consequently, LDH measurement is frequently used as a biochemical indicator of cytotoxicity, inflammation, and disease progression in experimental models.
Pathways
LDH catalyzes the reversible conversion of pyruvate to lactate during glycolysis, coupling this reaction with NADH oxidation to NAD+ regeneration. This step is essential for maintaining glycolytic flux under hypoxic conditions. LDH activity is closely linked to metabolic reprogramming pathways, particularly in rapidly proliferating cells and tumor microenvironments characterized by enhanced glycolysis.
For Research Use Only | Not For Clinical Use
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