CytoFinal™ Rat Lactate Dehydrogenase (LDH) ELISA Kit

CAT#: ITS-0326-WB105
Product Type: ELISA Kit
Species: Rat
Target: Lactate dehydrogenase (LDH) enzyme
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Accurately quantifies lactate dehydrogenase (LDH) levels in rat biological samples using a sensitive ELISA method.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat LDH ELISA Kit provides a high-precision, in vitro solution for measuring lactate dehydrogenase in rat serum, plasma, tissue lysates, and other biological fluids. Utilizing a sandwich ELISA format, this kit enables sensitive, reproducible quantification of LDH, facilitating studies of cellular metabolism, tissue damage, and disease biomarker profiling.
Features
High sensitivity detection permits accurate measurement of LDH activity in low-abundance rat samples with reproducible results.
Excellent specificity ensures minimal cross-reactivity with other dehydrogenases, improving assay accuracy and reliability.
Complete kit includes pre-coated plates, standards, antibodies, and reagents for consistent assay performance.
User-friendly colorimetric ELISA format allows straightforward quantification using standard microplate readers without specialized equipment.
Process Relevance
Monitors LDH release to assess tissue damage, cytotoxicity, and metabolic alterations in rat experimental models.
Application Stage
Drug development, preclinical toxicity studies, and in-process research biomarker monitoring in rat models.
Applications
Supports ELISA-based LDH quantification in serum, plasma, tissue lysates, and cell culture media, facilitating ADME profiling studies.
Target
Lactate dehydrogenase (LDH) enzyme
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cell metabolism, toxicology, hepatology, cardiovascular research, oncology, pharmacokinetics (ADME), pharmacology, and preclinical biomarker studies, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement using colorimetric ELISA with high precision and reproducibility.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.33 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase (LDH) catalyzes the reversible conversion of pyruvate to lactate, linking glycolysis and anaerobic metabolism. Elevated LDH levels indicate tissue damage, hypoxia, or metabolic stress, making it a widely used biomarker in preclinical research. Monitoring LDH in rat models provides insight into organ injury, drug toxicity, and cellular metabolic state.
Pathways
LDH is central to anaerobic glycolysis and lactate metabolism. It interconnects with pyruvate metabolism, the TCA cycle, and NAD+/NADH redox balance, influencing energy production and cellular stress responses in tissues.
For Research Use Only | Not For Clinical Use
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