CytoFinal™ Standard Mouse Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB90
Product Type: ELISA Kit
Species: Mouse
Target: Mouse cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects cytoplasmic AST (GOT1) levels in mouse serum, plasma, tissues, and cell lysates with reliable sensitivity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Standard Mouse AST (GOT1) ELISA Kit enables accurate, in vitro measurement of cytoplasmic AST across diverse mouse biological samples. Ideal for preclinical studies, biomarker validation, and metabolic research, it delivers reproducible results in serum, plasma, tissues, and cell lysates.
Features
Sandwich ELISA format with high-specificity antibodies ensures precise detection of cytoplasmic AST in multiple mouse sample types.
Sensitive detection with a wide dynamic range supports accurate quantification in various biological conditions.
Compatible with serum, plasma, tissue homogenates, and cell lysates, offering versatility for preclinical research applications.
Includes lyophilized standards and stable detection reagents to guarantee consistency and long-term usability across experiments.
Process Relevance
Facilitates accurate monitoring of cytoplasmic AST to assess liver function and metabolic activity in mouse models.
Application Stage
Applicable in preclinical research, disease modeling, and in-process laboratory quality control.
Applications
Quantitative analysis of AST activity in mouse serum, plasma, tissues, and cell lysates for biomarker discovery, hepatology studies, ADME profiling studies, and metabolic research applications.
Target
Mouse cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolic disease, enzymology, biomarker development, pharmacokinetics (ADME), preclinical pharmacology, translational research, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST activity in mouse biological samples.
Assay Range
0.16 ng/ml - 10 ng/ml
LOD
0.1 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 catalyzes the reversible transamination between aspartate and α-ketoglutarate, central to amino acid metabolism and cellular energy homeostasis. Its levels reflect hepatocellular integrity and metabolic function in preclinical studies.
Pathways
AST participates in the malate-aspartate shuttle, connecting cytoplasmic and mitochondrial redox states. Enzyme activity responds to amino acid availability and supports TCA cycle flux and energy production.
For Research Use Only | Not For Clinical Use
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