DBA/2 Mouse Splenocytes (CAT#: IC-LX060)

Containing ≥ 1 X 10*6 / vial lyophilized cells

>95%

>90%

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

DBA/2 Mouse Splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells.

Our immune cell are for research use only, not for diagnostic or therapeutic use.

Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Lyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles