DNA Quadruplex based DNA targeting Therapeutic Development Service
Introduction What We Can Offer Workflow Published Data Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Creative Biolabs offers specialized G4-based therapeutic development. We deliver validated G4 targets, optimized small molecule ligands, and robust preclinical data, enabling you to advance novel therapeutic candidates with confidence. Our expertise ensures that your project benefits from a deep understanding of G4 biology and cutting-edge methodologies, leading to more effective and specific drug development.
Introduction of DNA Quadruplex-based DNA-targeting Therapeutic Development Service
DNA quadruplexes (G4s) are four-stranded nucleic acid structures in guanine-rich genomic regions like telomeres and oncogene promoters. Their dynamic roles in fundamental cellular processes make them attractive therapeutic targets. Research on compounds such as EMICORON, telomestatin, and quarfloxin highlights G4 modulation's potential in treating cancer, viral, and antimicrobial diseases.
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What We Can Offer
Comprehensive & Customized Solutions
We offer end-to-end support, from precise target identification and validation to advanced preclinical development, all meticulously tailored to your specific project needs and scientific objectives. This ensures a holistic approach that adapts to the unique demands of each drug discovery program.
Precision Target Identification & Ligand Design
We accurately pinpoint and validate disease-relevant G4 structures using advanced bioinformatic analysis and biophysical characterization. Subsequently, we design and optimize highly selective, potent G4-binding small molecules via rational design, HTS, and FBDD.
Robust In-Cell & Preclinical Validation
We provide unparalleled insights into drug behavior in a physiologically relevant context. Furthermore, we leverage advanced preclinical models such as patient-derived xenografts (PDXs), orthotopic cancer models, and metastatic models for rigorous in vivo efficacy studies, generating robust and translationally relevant data.
Workflow of Quadruplex-Specific Therapeutic Development Service
Published Data
A significant advancement in the field of G-quadruplex-based technologies involves the creation of G-quadruplex DNA metalloenzymes. Figure 1 from a relevant publication illustrates this by showing the construction of a human telomeric G-quadruplex DNA metalloenzyme. Panel a provides a schematic of the molecular recognition between the terpyridine-Cu(II) complex (CuL1) and the human telomeric DNA sequence (HT21). Panels b and c present biophysical characterization data through CD spectra and UV melting curves, respectively. Furthermore, panels d and e detail an isothermal titration calorimetry (ITC) experiment, showcasing raw data and integrated calorimetric plots with thermodynamic parameters, demonstrating the binding events. This highlights how G4s can serve as scaffolds for catalytic activity, expanding their therapeutic and biotechnological potential beyond direct drug binding.
Fig.1 Construction of human telomeric G-quadruplex DNA metalloenzyme.1
Highlights
Unparalleled Expertise
Our team provides insightful guidance and innovative solutions, from initial concept to preclinical candidate. This deep specialization allows us to navigate the intricate complexities of G4 topologies, predict and mitigate off-target effects, and design optimal therapeutic pathways.
State-of-the-Art Platforms
We leverage a suite of advanced platforms, including computational tools for in silico modeling, high-throughput screening for rapid compound identification, and sophisticated analytical instruments for precise G4 analysis and ligand profiling.
Integrated & Efficient Approach
Our integrated approach unifies diverse disciplines and technologies to streamline the entire development process from discovery to preclinical validation. This minimizes bottlenecks and maximizes the potential of your therapeutic candidates.
Commitment to Quality & Client Success
Our solutions are designed to be adaptable, allowing us to pivot strategies based on new scientific findings or evolving project goals.
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Customer Reviews
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Exceptional Specificity
Creative Biolabs' service has been a game-changer for our lead optimization, enabling us to identify highly specific G4 ligands and avoid off-target effects that previously hindered our research. - Dr. L***a S.
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Accelerated Preclinical Validation
Creative Biolabs' comprehensive preclinical models and insightful ADME/Tox profiling accelerated lead validation. The robust data, especially from patient-derived xenografts, bridges the translational gap and provides critical early indicators that saved us significant time. - Dr. J***n R.
FAQs
What G-quadruplex structures can Creative Biolabs target?
We target a wide array of G-quadruplex structures, including those in telomeres, oncogene promoters (c-MYC, KRAS, BCL2), and viral genomes. Our advanced tools identify and validate G4s with diverse topologies relevant to various diseases. Discuss your specific target with our team for a tailored assessment.
What preclinical models does Creative Biolabs use for efficacy testing?
For translationally relevant data, we use advanced preclinical models including patient-derived xenografts (PDXs), orthotopic cancer models (e.g., colon cancer), and metastatic dissemination models. These closely mimic human diseases, providing robust efficacy data for your G4-targeting candidates.
What are typical G4-based drug development challenges, and how does Creative Biolabs address them?
Key challenges include achieving high G4/B-DNA specificity, optimizing cellular uptake/bioavailability, and predicting in vivo behavior. Creative Biolabs addresses these via an integrated platform: rational design, advanced in-cell validation, comprehensive ADME/Tox profiling, and sophisticated preclinical models, all guided by our scientific expertise.
Related Services
Structure Determination Service
Comprehensive protein expression, purification, and structural determination (X-ray crystallography, Cryo-EM, advanced NMR) to elucidate protein-ligand interactions.
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Preclinical Pharmacology
Our preclinical team designs and executes studies to assess drug absorption, distribution, metabolism, excretion, and the overall therapeutic effect in relevant in vivo systems, providing essential data.
How to Contact Us
Creative Biolabs' cutting-edge DNA quadruplex-based therapeutic development service is an integrated, expert-driven solution. Partner with us to unlock new therapeutic frontiers. Ready to accelerate your G4-based therapeutic development? Reach out to our expert team today. Contact us for more information and to discuss your project.
Reference
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Li, Yinghao, et al. "Terpyridine–Cu (II) targeting human telomeric DNA to produce highly stereospecific G-quadruplex DNA metalloenzyme." Chemical Science 6.10 (2015): 5578-5585. Distributed under an Open Access license CC BY 3.0, without modification. DOI: https://doi.org/10.1039/c5sc01381j
For Research Use Only | Not For Clinical Use
