Short Decsription
End-point chromogenic endotoxin test kit is conducted by adding a colorless artificial peptide substrate solution into mixture of lyophilized amebocyte lysate and test sample after a certain incubation period.
Description
End-point chromogenic endotoxin test kit is conducted by adding a colorless artificial peptide substrate solution into mixture of lyophilized amebocyte lysate and test sample after a certain incubation period.
Features
Highly sensitivity; Fast; Accurate; Broad application.
Applications
In vitro detection and quantitation of gram-negative bacterial endotoxins
Molecular Mechanism of Action
LAL test relys on factor C coagulation cascade found in horseshoe crabs' blood. The combination of endotoxin and zymogen factor C initiate the protease cascade. Next, the activated factor C goes onto activate factor B, which converts the proclotting enzyme to the clotting enzyme. Lastly, two peptide bonds in coagulogen are catalytically cleaved to form coagulin gel.
Qualified With
Quality certificate
Detection Method
Chromogenic
Method Type
Lyophilized amebocyte lysate (LAL)
Datasheet