Engineering Cancer Cells with IL-15 Overexpression for Stronger T Cell Activation
Many different novel strategies have been developed to derive genetically modified tumor cells and use them as cellular vaccines to induce useful antitumor immunity. Genetically modified tumor cells with IL-15 represent one of the novel promising approaches. With our cell line development services, scientists at Creative Biolabs are proficient at engineering tumor cell lines with IL-15 overexpression. We are confident to handle all aspects of your engineering tumor cell workflow, from start to finish.
IL-15 Functions in T Cell Activation
Interleukin (IL)-15 is a cytokine that promotes the development and homeostasis of a group of lymphocytes. IL-15 shares many functions in regulating both adaptive (stimulation of T cell proliferation and induction of cytotoxic T lymphocytes) and innate (activating natural killer (NK) cells) immune responses. IL-15 shares biological activities with IL-2. Additionally, unlike IL-2, IL-15 has several added benefits, including the lack of stimulation on regulatory T cell populations, reduced activation-induced cell death (AICD), and lower toxicity.
- IL-15 induces the activation, proliferation and survival of T cells.
- IL-15 induces the maintenance of high-avidity, antigen-specific CD8+memory T cells in the long term.
- IL-15 can also affect T-cell numbers by influencing T-cell trafficking.
- IL-15 regulates both proliferation and antibody production by activated B cells.
- IL-15 induces the development, persistence and activation of NK and NKT as well as γ/δT cells.
- IL-15 activates the dendritic cells and macrophages to produce pro-inflammatory cytokines that participate in T cell activation and differentiation.
Studies Performed with IL-15 Overexpressing Tumor Cells
- A study showed a whole-cell CT26 colon cancer vaccine, which secretes a complex of IL-15 and IL-15Rα, could trigger anti-tumor response via CD4 T, CD8 T, and NK cell-dependent cytotoxicity.
- A study indicated the human small cell lung cancer cell line, N592, genetically engineered to secrete IL-15, showed a reduced tumor growth rate.
Engineering Cancer Cells with IL-15 Overexpression Service
Designing specific active immunotherapy protocols utilizing cytokine gene-modified tumor cells extends the realm of cancer therapeutic possibilities. To promote the future of cancer immunotherapy, Creative Biolabs provides cell line engineering services to integrate the IL-15 gene into the host cell of your interest. Guaranteed by professionals with comprehensive experience, we offer a full package covering from vector design and cell line generation, to assay development, all serving to fulfill your needs. We have access to a range of gene transfer technologies and an expert team experienced at working with a variety of cancer cell lines, including adherent and suspension cells. We can provide small to large-scale custom cell lines with a fast turnaround and complete validation.
We help choose the most effective transfection method for your application and cell type, including:
Our cancer cell engineering services cover a range of different cell lines, including:
Creative Biolabs provides you with state-of-the-art cell line engineering and production services. Each project is customized for the desired deliverables by working directly with our team of highly trained scientists using a milestone-measured process. Please contact us for consultations on study design, choice of vector, and how we can enable your imagination!
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