Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide FaDu-based In Vitro MTT Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
FaDu
Target Cell Organism
Human
Target Cell Background
FaDu is a cell line with epithelial morphology that was established in 1968 from a punch biopsy of a hypopharyngeal tumor removed from a 56-year-old, White, male patient with squamous cell carcinoma. This cell line is a suitable transfection host and has applications in cancer and immuno-oncology research.
Related Diseases
Squamous Cell Carcinoma
Research Area
Oncology
Assay Name
In Vitro MTT Assay (Cell Viability)
Short Description
FaDu-cell based In Vitro MTT Assay (Cell Viability)
Assay Description
The MTT (3-(4,5-dimeth ylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay is one of most commonly used assays for cancer drug screening. This assay has been very popular among cancer researchers as an initial assay in drug discovery. NAD(P)H-dependent oxidoreductase or dehydrogenases in viable cells can reduce MTT into purple-colored formazan, which can then be solubilized for spectrophotometric analysis.
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins