Annexin V Apoptosis Assay

Annexin V assay is one of the most reliable methods to detect apoptosis with high accuracy and reliability. Annexin V is a protein that has a high affinity for phosphatidylserine, a molecule that translocates from the inner to the outer leaflet of the plasma membrane at the early stages of apoptosis. By binding to exposed phosphatidylserine, Annexin V, typically conjugated to a fluorescent marker, allows for the identification and quantification of apoptotic cells through flow cytometry. This method provides a clear and accurate measure of cell apoptosis, facilitating advanced research and therapeutic development.

Creative Biolabs, a distinguished biotechnology company, is dedicated to providing cutting-edge services and solutions in the field of biomedical research. Among our extensive portfolio, we offer the Annexin V apoptosis assay, a highly reliable and sensitive method for detecting cell apoptosis. Our assay employs flow cytometry to deliver precise quantification and analysis of apoptotic cells, thereby advancing research in cell biology, cancer studies, and drug development.

Our Services

At Creative Biolabs, our flow cytometry-based annexin V apoptosis assay involves the staining of cells with fluorescently labeled annexin V, which allows for the differentiation between apoptotic and non-apoptotic cells. Furthermore, propidium iodide (PI) or another DNA-binding dye can be used in tandem to distinguish between early apoptotic cells (annexin V+, PI-), late apoptotic or necrotic cells (annexin V+, PI+), and live cells (annexin V-, PI-), providing a comprehensive profile of cell health and viability. Flow cytometry quantifies the cells in various stages of apoptosis and necrosis based on their fluorescence properties.

Table 1 Analysis of Annexin V base-apoptosis assay. (Creative Biolabs original)

Our service begins with a detailed consultation to fully understand your unique research goals and experimental needs. Following this, our expert team customizes the annexin V apoptosis assay protocol, including the selection of appropriate controls and optimization of staining and flow cytometry parameters. The stained samples are then analyzed using state-of-the-art flow cytometry equipment, and the resulting data is interpreted to provide you with detailed, insightful results. Please contact us to discuss your apoptosis assay project further.

Highlights of Our Service

• High Sensitivity and Specificity: Our assays are designed to detect early and late stages of apoptosis with high sensitivity and specificity.
• Comprehensive Results: By employing dual staining with annexin V and viability dyes, we provide a comprehensive analysis of the cell population, distinguishing between live, apoptotic, and necrotic cells.
• Advanced Technology: Our advanced flow cytometry instrumentation enables high-resolution analysis and precise quantification of apoptotic cells, providing you with robust and reproducible data.
• Customized Solutions: We offer customized assay designs and protocols tailored to meet the specific needs and objectives of your project.

For Research Use Only | Not For Clinical Use