As a leader and expert in the field of oncology imaging research services, Creative Biolabs has completed a series of challenges in the past years. In particular, we have established an advanced gene-targeting lineage tracing discovery and analysis platform that enables us to offer a series of high-quality services for our worldwide customers.

Introduction to Gene-targeting Lineage Tracing

Studies in the past decades have shown that accurate interpretation of tumor cell fate plays an important role in elucidating the mechanisms and functions of tumor cell genesis, development, and regeneration. Gene-targeting lineage tracing has become a major approach to monitoring cell lineage and fate. Among them, recombination systems such as FLP/FRT, Cre-loxP, and Dre-rox have been widely studied. In general, these systems can be efficiently recombined, with high system specificity and no cross-reaction between systems, which is convenient for researchers to use flexibly.

As a professional lineage tracing research CRO company, Creative Biolabs has developed a comprehensive service chain in gene-targeting lineage tracing system discovery for tumor diagnosis. With in-depth expertise in molecular modeling, structural analysis, and in-vivo and in-vitro tumor lineage tracing studies, we can provide several site-specific recombination system solutions based on a wide collection of advanced equipment and technologies. We are dedicating ourselves to helping our worldwide customers shorten tumor lineage tracing analysis time in a low-cost manner.

Chief Services

• Fixed-point transformation
  In our labs, once the recombinant enzyme site has been generated at a certain location in a specific human genome, this site can be used as a target site for the insertion and expression of foreign genes, thus achieving site-specific transformation.
• Deletion of marker genes
  We can insert two enzyme sites in the same direction on both sides of the selected marker gene, and with the help of certain recombinases, the selected marker gene can be deleted.
• Gene/protein expression modification
  We have developed a way to control the expression of any gene at a particular time and in a particular type of cell. Up to now, we can achieve spatio-temporal specific expression of a single gene or the continuous expression of multiple genes in a single cell type.
• Genetically-modified mouse model construction
  We can construct a variety of mouse cancer models mediated by different recombinant systems, such as Cre-loxP and Dre-rox, to analyze the tumor microenvironment, evaluate the mechanisms of drug resistance, as well as optimize preclinical trials for tumor intervention.

Dual/Multiple DNA Recombinases Genetic Lineage Tracing Systems

To facilitate tracking tumor cells and improve the accuracy of lineage tracing, we developed a double-report and multiple-report gene lineage tracing system to reflect the different combinations of recombinant enzymes in different readouts.

In our lab, different loxP loci have been designed and their location/orientation has been controlled for dual cell labeling and cloning analysis, providing valuable information for cell fate mapping after cell proliferation and differentiation.

For genes that are not specific to specific cell types, we have developed a series of multiple recombinant enzyme-mediated genetic labeling systems to increase the specificity and number of cell types that are simultaneously labeled. For example, FLP/FRT, Cre-loxP, and Dre-rox are often used to design dual systems.

• Well-established Dual/Multiple DNA Recombinase platform
• Fully integrated lineage tracing services
• Cost-saving discovery strategy

Lineage tracing has played an important role in many disease research, especially in various cancers. As a top bio-company in novel tumor lineage tracing development, Creative Biolabs is dedicated to offering a wide spectrum of lineage tracing systems to illustrate tumor cell bioactivity and mechanism of action. If you are interested in our services, please contact us or directly send us an inquiry.

For Research Use Only | Not For Clinical Use