GIST-T1 In Vitro Fluorescence Microscopy-based Apoptosis Assay

This assay is to provide GIST-T1-based In Vitro Fluorescence Microscopy-based Apoptosis Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

GIST-T1

Human

A new human cell line, GIST-T1, was established from a metastatic plural tumor from a gastrointestinal stromal tumor (GIST) of the stomach in a Japanese woman, and was characterized by immunohistochemistry, conventional banding methods, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH).

Gastrointestinal Stromal Tumor; GIST

Oncology

In Vitro Fluorescence Microscopy-based Apoptosis Assay

GIST-T1-cell based In Vitro Fluorescence Microscopy-based Apoptosis Assay

Excellent signal-to-noise ratios and good temporal and spatial resolutions in fluorescence microscopy have made this technique one of the most widely used microscopic techniques in cancer research. Owing to high spatial resolution in fluorescence microscopy, this has become an excellent technique to observe cell and tissue imaging and biological processes and unlike the other techniques (light microscopy and mass spectrometry), this technique has a good level of user selectivity.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Microscopic Techniques to Detect Apoptosis Assays

Fluorescence Microscopy Assay