The kit is designed for in vitro quantitative measurement of Goat IFNA in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.
Description
This ELISA kit is a solid phase ELISA designed for quantitative determination.
Applications
ELISA
Application Notes
The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. Please predict the concentration before assaying. If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary. Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits, some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products. Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit. Fresh samples without long time storage are recommended for the test.
Microtiter plate (96 wells stripwell) - 1 Enzyme conjugate - 1 vial Standard A - 1 vial Standard B - 1 vial Standard C - 1 vial Standard D - 1 vial Standard E - 1 vial Standard F - 1 vial Substrate A - 1 vial Substrate B - 1 vial Stop solution - 1 vial Wash solution - 1 vial Balance solution - 1 vial Instruction manual - 1
Material not included
Precision pipettors and disposable tips to deliver 10-1000 μL. A multi-channel pipette is desirable for large assays. 100 mL and 1 L graduated cylinders. Distilled or deionized water. Tubes to prepare sample dilutions. Absorbent paper. Microplate reader capable of measuring absorbance at 450 nm. Centrifuge capable of 3000 x g. Microplate washer or washing bottle. Incubator (37 °C). Data analysis and graphing software.