In the rapidly advancing fields of drug discovery and biological research, the capability to efficiently screen therapeutic antibodies and proteins is crucial. As a leader in the biotechnology industry, Creative Biolabs provides an innovative GPCR panel screening service, paving the way for groundbreaking developments in therapeutic antibody and protein research.

Introduction

G protein-coupled receptors (GPCRs) are a vast and diverse group of membrane proteins that play critical roles in cellular signal transduction. These receptors work by transmitting signals through G-proteins, affecting calcium and cAMP secondary messenger pathways. They are pivotal in various physiological processes, from sensory perception to immune response, and are implicated in numerous diseases, making them a central focus for therapeutic target discovery. GPCRs comprise about 33% of all drug targets, underscoring their medical relevance.

Fig.1 Structure, function and drug discovery of GPCR signaling.^1,2

GPCR Panel Screening Service

At Creative Biolabs, we leverage our expertise in cellular biology and pharmacology to offer unparalleled GPCR panel screening services tailored for the development of therapeutic antibodies and proteins. Our service is designed to foster the discovery and validation of potential therapeutic compounds that harness the functionality of GPCRs.

Our comprehensive service encompasses the high-throughput screening of compounds targeted at GPCRs and robust data analysis to evaluate compound efficacy, specificity, and mechanism of action. Collaborating with clients, we utilize state-of-the-art technologies to facilitate rapid and informed decision-making in the drug discovery process.

Features of the GPCR Panel

• Broad Target Coverage: Our GPCR panel comprises over 170 GPCR overexpression stable cell lines and covers a broad array of targets such as 5-Hydroxytryptamine, acetylcholine, dopamine, glucagon, and opioid receptors. With this extensive selection, we can tailor the screening process to align with clients' specific research objectives, whether for neurological, cardiovascular, or metabolic diseases, among others.
• Binding Affinity Validation: To ensure comprehensive characterization, our panel includes SPR binding assays, tag-lite binding assays, and FACS binding assays. These are configured to validate the affinity and interaction between GPCRs and potential drug compounds.
• Functional Assays for In-Depth Analysis: The panel incorporates a variety of functional assays, such as cAMP assays, calcium flux assays, IP1 assays, and reporter assays. These assays enable us to monitor the production of secondary messengers and dissect the GPCR-mediated signaling pathways in detail.
• High-throughput Screening: Creative Biolabs integrates high-throughput screening capabilities with advanced analytical methods, enabling large-scale screening operations that deliver rapid and precise results, essential for lead compound identification and optimization.
• Stable Cell Lines for Consistency: Each GPCR target within our panel is expressed in stable, clonal cell lines, ensuring consistency and reproducibility across experiments. This stability is critical for establishing reliable protocols and generating actionable data for compound profiling.

Why Choose Us?

• Comprehensive Target Coverage
  Creative Biolabs offers a broad GPCR panel with over 170 stable cell lines, covering critical targets such as serotonin, acetylcholine, dopamine, and opioid receptors, ensuring tailored solutions for diverse research needs.
• High-Throughput Screening
  Our high-throughput screening technology allows for rapid and efficient evaluation of large compound libraries, accelerating the identification and optimization of lead drug candidates.
• Advanced Functional Assays
  With functional assays such as cAMP and calcium flux, we provide in-depth analysis of GPCR-mediated signaling pathways, helping to uncover detailed compound mechanisms and efficacy.
• Reliable and Consistent Results
  Using stable, clonal cell lines for each GPCR target, we ensure reproducibility and consistency in experimental data, which is crucial for accurate drug development and profiling.

Contact us today to discover how our services support your drug discovery endeavors.

Reference

1. Cheng, Lin, et al. "Structure, function and drug discovery of GPCR signaling." Molecular Biomedicine 4.1 (2023): 46. under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use