GuardianOx™ Antioxidant Assay Kit

CAT#: ITS-1125-YL31
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Measures total antioxidant capacity using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Antioxidant Assay Kit offers a comprehensive colorimetric method for evaluating the total antioxidant capacity of biological fluids and experimental samples. The system measures the reduction of metal ions by antioxidant molecules, providing a reliable index of radical scavenging activity. This essential tool facilitates research into oxidative stress mechanisms and cellular defense systems, ensuring high sensitivity and reproducible results for fundamental studies in redox biology.
Features
Measures total antioxidant capacity via a standardized colorimetric reduction assay, providing a comprehensive assessment of oxidative stress resistance in various biological research models.
Process Relevance
Evaluating total antioxidant capacity to monitor cellular defense mechanisms against oxidative stress in vitro.
Application Stage
Oxidative stress research and antioxidant screening.
Applications
Determine total antioxidant capacity in biological samples to evaluate cellular defense mechanisms against oxidative stress and free radical damage.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570nm
Sample Type
Serum, plasma, urine, saliva, food, plant extracts.
Research Areas
Oxidative Stress Research; Aging Research; Pharmacology
Size
100 tests
Data Output Type
Total Antioxidant Capacity (TAC) value
Assay Time
20 min
LOD
LOD: 1.5 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
The total antioxidant capacity of a biological sample reflects its ability to neutralize reactive oxygen species and mitigate oxidative stress. This parameter is a vital indicator of the overall redox status and health of cells and tissues.
Pathways
GSH is a key substrate for glutathione peroxidase in the neutralization of hydrogen peroxide. It acts as an upstream regulator of the cellular redox state and is essential for the detoxification of electrophilic xenobiotics.
For Research Use Only | Not For Clinical Use
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