GuardianOx™ Carbonyl Assay Kit

CAT#: ITS-1125-YL32
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Quantifies protein carbonyls using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Carbonyl Assay Kit provides a robust colorimetric method for quantifying oxidative damage to proteins in biological samples. By measuring the concentration of carbonyl groups through reaction with dinitrophenylhydrazine, the system offers a reliable index of protein oxidation. This specialized tool is vital for researchers investigating oxidative stress pathways and aging mechanisms, providing consistent performance for fundamental studies in cellular damage and repair.
Features
Detects protein carbonyl groups through a specific chemical derivatization, providing a sensitive and reliable biomarker for monitoring oxidative protein damage in research on aging.
Process Relevance
Quantifying protein carbonylation to assess oxidative damage and evaluate protein stability in research studies.
Application Stage
Protein oxidation studies and oxidative stress monitoring.
Applications
Quantify protein carbonyl groups in tissue homogenates and serum to assess oxidative damage and protein degradation in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 375nm
Sample Type
Cell lysates, tissue homogenates, protein samples.
Research Areas
Protein Oxidation; Oxidative Stress; Gerontology
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 12 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Protein carbonylation is a widespread and irreversible marker of oxidative damage to proteins. Monitoring these modifications is essential for understanding the impact of oxidative stress on protein function and cellular aging.
Pathways
NO is synthesized by nitric oxide synthases (NOS) and acts as an upstream activator of soluble guanylyl cyclase, leading to the production of cGMP. It is a critical mediator in downstream signaling pathways controlling vascular tone and synaptic plasticity.
For Research Use Only | Not For Clinical Use
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