Short Description
Quantifies protein carbonyls using a colorimetric method.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Carbonyl Assay Kit provides a robust colorimetric method for quantifying oxidative damage to proteins in biological samples. By measuring the concentration of carbonyl groups through reaction with dinitrophenylhydrazine, the system offers a reliable index of protein oxidation. This specialized tool is vital for researchers investigating oxidative stress pathways and aging mechanisms, providing consistent performance for fundamental studies in cellular damage and repair.
Features
Detects protein carbonyl groups through a specific chemical derivatization, providing a sensitive and reliable biomarker for monitoring oxidative protein damage in research on aging.
Process Relevance
Quantifying protein carbonylation to assess oxidative damage and evaluate protein stability in research studies.
Application Stage
Protein oxidation studies and oxidative stress monitoring.
Applications
Quantify protein carbonyl groups in tissue homogenates and serum to assess oxidative damage and protein degradation in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 375nm
Sample Type
Cell lysates, tissue homogenates, protein samples.
Research Areas
Protein Oxidation; Oxidative Stress; Gerontology
Datasheet