Short Description
Quantifies glutathione levels using a colorimetric method.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Glutathione (GSH) Assay Kit provides a sensitive colorimetric method for measuring total and reduced glutathione levels in biological samples. Utilizing an enzymatic recycling reaction, the system amplifies the signal to allow for the quantification of low concentrations. This essential tool is vital for researchers investigating cellular redox status and antioxidant defense mechanisms, offering high sensitivity and reproducibility for fundamental studies in oxidative stress.
Features
Features a high-specificity enzymatic recycling method for quantifying GSH and GSSG, enabling the precise determination of intracellular redox status and oxidative stress in research.
Process Relevance
Quantifying glutathione levels to evaluate intracellular redox status and antioxidant defense capacity in research.
Application Stage
Redox signaling and oxidative stress analysis.
Applications
Measure reduced and total glutathione levels in cell lysates to investigate cellular redox homeostasis and oxidative stress responses.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412nm
Sample Type
Whole blood, cell lysates, tissue homogenates.
Research Areas
Redox Homeostasis; Toxicology; Cell Signaling
Datasheet