GuardianOx™ Glutathione (GSH) Assay Kit

CAT#: ITS-1125-YL34
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Quantifies glutathione levels using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Glutathione (GSH) Assay Kit provides a sensitive colorimetric method for measuring total and reduced glutathione levels in biological samples. Utilizing an enzymatic recycling reaction, the system amplifies the signal to allow for the quantification of low concentrations. This essential tool is vital for researchers investigating cellular redox status and antioxidant defense mechanisms, offering high sensitivity and reproducibility for fundamental studies in oxidative stress.
Features
Features a high-specificity enzymatic recycling method for quantifying GSH and GSSG, enabling the precise determination of intracellular redox status and oxidative stress in research.
Process Relevance
Quantifying glutathione levels to evaluate intracellular redox status and antioxidant defense capacity in research.
Application Stage
Redox signaling and oxidative stress analysis.
Applications
Measure reduced and total glutathione levels in cell lysates to investigate cellular redox homeostasis and oxidative stress responses.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412nm
Sample Type
Whole blood, cell lysates, tissue homogenates.
Research Areas
Redox Homeostasis; Toxicology; Cell Signaling
Size
250 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 12 µg/dL (0.4 µM)
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This tripeptide is the most abundant intracellular antioxidant and plays a central role in protecting cells from oxidative damage and regulating the redox environment. The ratio of reduced to oxidized glutathione is a sensitive indicator of cellular health and stress.
Pathways
Peroxides are primary reactive oxygen species produced as byproducts of mitochondrial respiration and by enzymes like NADPH oxidase. They serve as upstream signaling molecules in the activation of redox-sensitive transcription factors like Nrf2.
For Research Use Only | Not For Clinical Use
Online Inquiry