GuardianOx™ TBARS Assay Kit

CAT#: ITS-1125-YL38
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Measures lipid peroxidation using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ TBARS Assay Kit provides a standardized colorimetric method for quantifying malondialdehyde, a key marker of lipid peroxidation. The system measures the formation of a colored adduct under acidic conditions, offering a reliable index of oxidative damage to lipids. This robust tool facilitates research into oxidative stress mechanisms and membrane integrity, ensuring consistent results for fundamental studies in cellular physiology and aging.
Features
Measures malondialdehyde levels through a standardized TBARS reaction, providing a reliable and sensitive marker for quantifying lipid peroxidation and oxidative membrane damage in research.
Process Relevance
Quantifying lipid peroxidation to assess oxidative stress levels and evaluate membrane damage in vitro.
Application Stage
Lipid peroxidation research and oxidative stress monitoring.
Applications
Measure lipid peroxidation markers in biological samples to evaluate oxidative stress and cellular damage in various research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 535nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, urine, cell lysates, tissue homogenates.
Research Areas
Lipid Peroxidation; Oxidative Stress Research; Cardiovascular Research
Size
100 tests
Data Output Type
MDA equivalents
Assay Time
80 min
LOD
LOD: 0.1 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Thiobarbituric Acid Reactive Substances (TBARS) are widely used markers of lipid peroxidation and oxidative stress. Monitoring these end-products provides insight into the extent of oxidative damage to cellular and mitochondrial membranes.
Pathways
D-amino acids, such as D-serine, act as potent co-agonists for NMDA receptors in the brain. They are involved in the upstream modulation of synaptic plasticity and excitatory neurotransmission pathways.
For Research Use Only | Not For Clinical Use
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