Hbl-2 In Vitro Cellular Senescence Assay (Senescence) (CAT#: ITS-0123-YF3618)

Hbl-2

Human

The HBL-2 cell line is derived from an AIDS-SNCCL (AIDS-associated small non-cleaved cell lymphoma) patient. After immunophenotypic and molecular genetic analysis, the HBL-2 was established from the original tumor clones. The HBL-2 cell line presents surface immunoglobulin and B-cell restricted markers as well as a phenotype consistent with SNCCL; the cell line also displays clonal immunoglobulin gene rearrangement. The HBL-2 cell line is considered useful as a biological model to study AIDS-associated non-Hodgkin lymphomagenesis (AIDS-NHL) and the impacts of biological, immunological, and viral factors involved.

Mantle Cell Lymphoma; MCL

Oncology

In Vitro Cellular Senescence Assay (Senescence)

Although numbers of various oncogenes and tumor suppressor genes have been proven to regulate senescence in normal cells, evading cellular senescence appears to be a significant step in cancer. Lysosomal senescence-associated β-galactosidase (SA-β-gal) is one of the common markers used to identify
senescent cells. Senescence-associated secretory phenotype (SASP) secretion is a characteristic of senescent cells and several cytokines (interleukins 6 and 8), proteases (stromelysin-1), growth factors (vascular endothelial growth factor [VEGF]) and ECM components are some of the examples for SASPs. During early stages, SASP components help to promote migration of effector immune cells, tissue repair and release of proteases and growth factors. p53, pRb signaling (ALDH1A3, CCNA2 and AKT1) and some cell cycle genes (CDK4, CDK6 and CDK4) have been identified as genes controlling cellular senescence.

Cellular Senescence in Cancer Assays

Cell senescence is described as an irreversible physiological event of cell growth arrest, which is known to be caused by alterations of telomeres, oxidative stress, DNA damage and some oncogenes. Inhibition of the program of senescence is one of the main features of tumor cells and it has been reported that cancer cells can be induced to undergo senescence by chemo- and radiotherapies and genetic alterations. Telomeres help to prevent uncontrolled cell proliferation and the absence of telomeres results in aneuploidy and cell death.

Cellular Senescence Assay