Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HCC827-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HCC827
Target Cell Organism
Human
Target Cell Background
HCC827 is an epithelial cell that was isolated from the lung of a White, 39-year-old female patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna in 1994 and can be used in immuno-oncology research.
Related Diseases
Lung Cancer
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent sig nal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.