HCT-116 In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

This assay is to provide HCT-116-based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HCT-116

Human

HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

HCT-116-cell based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. In the DPPH assays, DPPH free radical (DPPH) accepts protons from compounds with antioxidant properties to form yellow colored stable DPPH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay