HCT-116 In Vitro MTT Assay (Cell Viability)

This assay is to provide HCT-116-based In Vitro MTT Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HCT-116

Human

HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro MTT Assay (Cell Viability)

HCT-116-cell based In Vitro MTT Assay (Cell Viability)

The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay is one of most commonly used assays for cancer drug screening. This assay has been very popular among cancer researchers as an initial assay in drug discovery. NAD(P)H-dependent oxidoreductase or dehydrogenases in viable cells can reduce MTT into purple-colored formazan, which can then be solubilized for spectrophotometric analysis.

MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins