HCT116 In Vitro S-nitrosylation Assay (Oxidative Stress)

This assay is to provide HCT116-based In Vitro S-nitrosylation Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HCT116

Human

HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be d as a positive control for PCR assays of mutation in this codon.

HCT 116

Colon Cancer

Oncology

In Vitro S-nitrosylation Assay (Oxidative Stress)

HCT116-cell based In Vitro S-nitrosylation Assay (Oxidative Stress)

S-nitrosylation (attachment of NO to thiol side chains of the amino acid cysteine) and S-glutathionylation (attachment of GSH to thiol side chains of the amino acid cysteine) are two post-translational modification processes reported to increase with oxidative stress.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances