Our Histone Deacetylase (HDAC) Panel Screening Service offers a specialized platform for identifying inhibitors of HDAC enzymes, a family of proteins responsible for removing acetyl groups from histone proteins and other non-histone substrates. Our comprehensive screening service allows researchers to screen large compound libraries or biologics, identify potent HDAC inhibitors, and explore their therapeutic potential in a variety of disease models.

Histone Deacetylase (HDAC) Overview

HDACs are important modulators of gene expression and chromatin structure. By removing acetyl groups from histones and other proteins, HDACs reverse acetylation, a post-translational modification that is generally associated with gene silencing. The acetylation-deacetylation cycle plays a critical role in cellular processes such as DNA repair, transcription, cell cycle regulation, and apoptosis. There are 18 known human HDACs, classified into four main classes:

• Class I (HDAC1, HDAC2, HDAC3, HDAC8): Mainly found in the nucleus, these proteins regulate the expression of genes.
• Class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, HDAC10): Shuttles between the nucleus and cytoplasm, with roles in development and disease.
• Class III (Sirtuins, SIRT1-7): NAD+-dependent HDACs that control aging, stress response, and cellular metabolism.
• Class IV (HDAC11): Shares similarities with both Class I and III HDACs, with a yet to be fully understood role in cellular processes.

Histone Deacetylase (HDAC) Panel Screening Service at Creative Biolabs

We offer:

Our HDAC Panel Screening Service provides comprehensive assays for the screening of HDAC inhibitors across multiple classes.

• Broad HDAC Panel: A panel covering the most commonly studied HDAC enzymes from Class I, II, and IV, including HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, and HDAC11, as well as Class III sirtuins (SIRT1-7).
• Enzyme Activity Assays: Measure the enzymatic activity of HDACs by detecting the deacetylation of histone substrates or synthetic peptides, typically using fluorescence or colorimetric methods.
• Inhibition Assays: Test compounds for their ability to inhibit HDAC activity by measuring the reduction in deacetylase activity in the presence of small molecules or biologics.
• Cell-Based Assays: Evaluate the functional effects of HDAC inhibitors in cultured cells, such as changes in histone acetylation, gene expression, cell cycle progression, and apoptosis. These assays are designed to mimic physiological conditions and assess the compound's potential in vivo.
• Selectivity and Potency Profiling: Assess the specificity and potency of compounds against individual HDAC isoforms, helping to identify the most selective inhibitors and determine the dose-response relationship.

Service process:

Why Choose Us

Frequently Asked Questions

A1: Which HDAC isoforms are included in your screening panel?

Q1: Our panel includes HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, and HDAC11 from Class I, II, and IV, as well as sirtuins (SIRT1-7) from Class III. Additional HDACs can be included upon request.

A2: What data will I receive from the screening?

Q2: You will receive a detailed report with IC50 values, dose-response curves, inhibition profiles, and selectivity information, helping you identify the most promising HDAC inhibitors for your research.

Contact Us

We invite you to connect with our specialized team for any inquiries related to our Histone Deacetylase (HDAC) Panel Screening Service. Whether you need detailed information about our testing methods, sample requirements, or data analysis, our dedicated experts are here to assist you. Your research is our priority, and we look forward to collaborating with you to advance your studies on HDAC dynamics and their implications in various biological contexts.

For Research Use Only | Not For Clinical Use