HEL In Vitro Catalase Assay (Oxidative Stress)

This assay is to provide HEL-based In Vitro Catalase Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HEL

Human

A new human erythroleukemia cell line has been established. This line, designated HEL, is capable of spontaneous and induced globin synthesis, producing mainly Gγ and Aγ chains. Embryonic chains (ε, ζ) and α chains are detectable in very small amounts; β chains are undetectable. This line provides a new model system for studying aspects of erythroid cell differentiation and differential globin gene expression.

Erythroleukemia

Oncology

In Vitro Catalase Assay (Oxidative Stress)

HEL-cell based In Vitro Catalase Assay (Oxidative Stress)

Wide ranges of enzyme assays are also available to detect oxidative stress at the cellular level. Enzymes such as NO synthases and xanthine oxidase are known to generate ROS. Enzymes namely superoxide dismutase, catalase and thioredoxin reductase can prevent cells from oxidative damage. GST is another enzyme involved in oxidative stress that catalyzes the reduction of oxidized GSH to GSH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Enzyme Assay