Target Cell Alternative Name: Hep-3B; Hep3B; Hep 3B2.1-7
Target Cell Name: Hep 3B
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Hep 3B-based In Vitro SRB Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Hep 3B
Target Cell Organism
Human
Target Cell Background
Hep 3B is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.
Target Cell Alternative Name
Hep-3B; Hep3B; Hep 3B2.1-7
Related Diseases
Hepatocellular Carcinoma
Research Area
Oncology
Assay Name
In Vitro SRB Assay (Cell Viability)
Short Description
Hep 3B-cell based In Vitro SRB Assay (Cell Viability)
Assay Description
The sulforhodamine B is another common colorimetric assay that is used to investigate viability of cancer cells. The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500-540 nm.
Assay Alternative Names
Sulforhodamine B Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins