HL-60 In Vitro Trypan Blue Exclusion Assay (Cell Viability)
CAT#: ITS-1122-YF3691
Target Cell Organism: Human
Target Cell Alternative Name: HL60
Target Cell Name: HL-60
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HL-60-based In Vitro Trypan Blue Exclusion Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HL-60
Target Cell Organism
Human
Target Cell Background
The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center.
Target Cell Alternative Name
HL60
Related Diseases
Leukemia
Research Area
Oncology
Assay Name
In Vitro Trypan Blue Exclusion Assay (Cell Viability)
Short Description
HL-60-cell based In Vitro Trypan Blue Exclusion Assay (Cell Viability)
Assay Description
The intact cell membranes cannot recruit these dyes into cells. Trypan blue can enter into non-viable cells, while viable cells exclude trypan blue. The trypan blue exclusion assay is a simple and commonly used assay for cell viability determination.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.