Horse CCL5 ELISA Kit, Lot 21AO-859 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Horse CCL5 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Equine RANTES ELISA Kit.

ELISA

Optimal working dilution should be determined by the investigator.

CCL5

Horse

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

6 months

For Research Use only

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

Rantes; Scya5; D17S136E; RANTES; SCYA5; SIS-delta; SISd; TCP228; eoCP; MuRantes; C-C motif chemokine 5-like; C-C motif chemokine ligand 5; chemokine (C-C motif) ligand 5; LOC100230935; CCL5; Ccl5

100033925

Q8MKD0

Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.