Horse TGFB1 ELISA Kit, Lot 21AO-1133 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1044
Product Type: ELISA Kit
Target: TGFB1
Short Description
The kit is designed for in vitro quantitative measurement of Horse TGFB1 in Plasma, Serum.
Description
For the quantitative determination of horse transforming growth factor beta-1(TGFB1) concentrations in serum, plasma.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
TGFB1
Reactivity
Horse
Detection Method
Colorimetric
Method Type
Competition ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum
Specificity
This assay has high sensitivity and excellent specificity for detection of horse TGFB1. No significant cross-reactivity or interference between horse TGFB1 and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between horse TGFB1 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
1.56 pg/mL
Sample Volume
50 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C.
Restrictions
For Research Use only
Alternative Name
Transforming Growth factor beta1
Synonyms
CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta; TGF-BETA-1; TGF-beta5; ced; dpd1; lap; tgf-beta; tgfb; tgfb5; tgfbeta; TGF-beta1; TGFbeta1; Tgfb; Tgfb-1; ai39657; tgfb1; wu:fb13a07; xx:ai39657; TGFB1; csd; cdb1; cdg2; csd1; csd2; csd3; ebmd; lcd1; bigh3; cdgg1; betaig-h3; TGFB4; transforming growth factor beta 1; transforming growth factor beta-1; transforming growth factor beta 1 L homeolog; transforming growth factor; beta 1; transforming growth factor; beta 1a; transforming growth factor beta induced L homeolog; TGFB1; Tgfb1; tgfb1.L; tgfb1a; tgfbi.L
Background
Abbreviation: TGFB1
Alias: CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide
Gene ID
100033900
UniProt
O19011
Pathways
EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
Online Inquiry