HPLC-MS/MS based Cytochrome P450 2D6 (CYP2D6) Inhibition Assessment Service
Our HPLC-MS/MS-based CYP2D6 Inhibition Assessment Service offers a comprehensive analysis of drug metabolism and drug interaction potential through the precise quantification of CYP2D6 enzyme activity. By utilizing state-of-the-art technology, our service provides accurate and reliable results to help researchers evaluate the inhibitory effects of compounds on the CYP2D6 enzyme.
HPLC-MS/MS-based CYP Inhibition Assessment Platform
HPLC-MS/MS-based Cytochrome P450 2D6 (CYP2D6) Inhibition Assessment Service
CYP2D6
CYP2D6 is an enzyme that is responsible for metabolizing a wide range of drugs. This enzyme plays a crucial role in determining how an individual's body processes and responds to certain drugs. Genetic variations in the CYP2D6 gene can result in differences in enzyme activity, leading to variations in drug metabolism and potential differences in drug response and efficacy. Testing for CYP2D6 gene variants may be recommended in certain cases to help guide drug therapy and prevent adverse reactions.
What We Do
Our service is utilized for assessing the inhibition of the CYP2D6 enzyme using a combination of high-performance liquid chromatography and tandem mass spectrometry.
- Sample preparation
The test compound is dissolved in an appropriate solvent and prepared at different concentrations for analysis.
- HPLC-MS/MS analysis
The prepared samples are injected into an HPLC system coupled with an MS/MS detector. The HPLC separates the compounds in the sample based on their chemical properties, and the MS/MS detector detects and quantifies the target compound.
- Incubation with CYP2D6 enzyme
The test compound is incubated with the CYP2D6 enzyme, which is a key enzyme responsible for drug metabolism in the liver. The enzyme metabolizes the compound, and its inhibitory effects on CYP2D6 activity are assessed.
- Data analysis
The data obtained from the HPLC-MS/MS analysis is analyzed to determine the inhibitory effects of the test compound on CYP2D6 activity. The IC50 value, which represents the concentration of the compound required to inhibit half of the enzyme activity, is calculated.
Detailed Information of Our Service
- CYP2D6 Information
| Protein Name | Family | Sub Family | Uniprot Number | Gene Name |
| CYP2D6 | CYP450 | CYP2 | P10635 | CYP2D6 |
| Gene ID | Gene Aliases | Organism | Construct Details | Tissue |
| 1565 | CPD6|P450-DB1|CYP2D|P450C2D | Human | Microsomes | Liver |
- Assay Information
Service Type
Biochemical
Function Mode
Antagonist
Measured Response
Peak Area Response
Service Sub Type
Enzymatic
Detection Method
HPLC-MS/MS
Substrate
Dextromethorphan
Applications of CYP2D6 Inhibition Assessment
- Identify individuals who may be at risk for adverse drug reactions or therapeutic failure when prescribed medications that are metabolized by this enzyme.
- Predict possible drug interactions when multiple drugs metabolized by the enzyme are administered simultaneously.
- Understanding the impact of CYP2D6 inhibition on drug metabolism and pharmacokinetics is useful to study results accurately and ensure the safety of the study.
- Identifying potential drug candidates that may be affected by genetic variations in this enzyme.
Cooperation With Creative Biolabs
Contact Us
For more information about our HPLC-MS/MS-based Cytochrome P450 2D6 (CYP2D6) Inhibition Assessment Service, or to inquire about pricing and turnaround time, please don't hesitate to contact us. In addition, we also provide you with Soft Spot Identification Service and In Vitro Cross Species Comparison Service. If you are interested in any other ADME services, please feel free to contact us!
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