Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HT1080-based In Vitro Resazurin Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HT1080
Target Cell Organism
Human
Target Cell Background
HT-1080 human cells are epithelial cells derived from connective tissue from a patient with Fibrosarcoma that can be used in research.
Target Cell Alternative Name
HT 1080
Related Diseases
Fibrosarcoma
Research Area
Oncology
Assay Name
In Vitro Resazurin Assay (Cell Viability)
Short Description
HT1080-cell based In Vitro Resazurin Assay (Cell Viability)
Assay Description
Resazurin is a non-fluorescent (blue in color) component of alamar Blue reagents, which can enter viable cells. After entering in to viable cells, resazurin is converted to resorufin by NADH, NAD (P) H or FADH2-dependent cellular oxidoreductase.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins