Human CD137 ELISA Kit, Lot 21AO-563 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human CD137 in Cell Lysate, Serum, Plasma.

Human 4-1BB-R ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human 4-1BB-R concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme- Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.

ELISA

CD137 (TNFRSF9)

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Lysate, Serum, Plasma

The Human 4-1BB-R ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human 4-1BB-R proteins.

The Human 4-1BBR ELISA is capable of recognizing both recombinant and naturally produced Human 4-1BBR proteins. The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. Human: 4-1BBL, AITRL, BAFF, BAFF Receptor, BCMA, CD40 Ligand/TRAP, Fas Receptor, LIGHT, OPG, sOX40L, sRANK (Receptor), sRANKL, TACI, TL-1A, TNF-α, TNF-β, sTNF-Receptor Type I, sTNF- Receptor Type II, sTRAIL/Apo2L, sTRAIL Receptor-1, sTRAIL Receptor-2, TWEAK, TWEAK Receptor.

1.Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips.
2.Protein Standard: Lyophilized (100 ng), Red container.
3.Biotinylated Detection Antibody: Lyophilized, Yellow container.
4.400x Streptavidin-HRP: 30 μL, Blue container.
5.Wash Buffer (10x): 50 mL, Clear containter.
6.Assay Diluent: 50 mL, Clear container.
7.Ready-to-Use Substrate: 12 mL, Brown container.
8.Stop Solution: 12 mL, Clear container.
9.Adhesive Plate Sealers: 4 Sheets.
10.Technical Manual 1 Manual.

Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm).
Micropipettes with capability of measuring volumes ranging from 1 μl to 1 Ml.
Deionized or sterile water.
Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer.
Graph paper or computer software capable of generating or displaying logarithmic functions.
Absorbent paper or vacuum aspirator.
Test tubes or microfuge tubes capable of storing ≥1 mL.
Bench.
top centrifuge (optional).
Bench.
top vortex (optional).
Orbital shaker (optional).

Pre-coated

If possible, all incubation steps should be performed on an orbital shaker to equilibrate solutions when added to the microplate wells. Also, all provided solutions should be at ambient temperature prior to use.

1.Average the duplicate or triplicate readings for each standard, control and sample and subtract the average zero standard optical density.
2.Generate a standard curve by using Microsoft Excel or other computer software capable of establishing a 4- Parameter Logistic (4-PL) curve fit.

Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.

This ELISA kit is intended for research purposes only, NOT diagnostic or clinical procedures of any kind.
Materials included in this kit should NOT be used past the expiration date on the kit label.
Reagents or substrates included in this kit should NOT be mixed or substituted with reagents or substrates from any other kits.

4 °C

Unopened Kits: Store at 4 °C for 6 months.
Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C.
Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

Unopened Kits: Store at 4 °C for 6 months.
Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C.
Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

For Research Use only

Tumor necrosis factor receptor superfamily member 9

TNFRSF9; zgc:136557; 4-1BB; CD137; CDw137; ILA; A930040I11Rik; AA408498; AI325004; Cd137; Ly63; tumor necrosis factor receptor superfamily member 9; TNF receptor superfamily member 9; tumor necrosis factor receptor superfamily; member 9a; tumor necrosis factor receptor superfamily; member 9; TNFRSF9; tnfrsf9a; Tnfrsf9

Human 4-1BB Ligand Receptor, also known as the Tumor Necrosis Factor Receptor Superfamily Member 9, is a 255 amino acid cytokine protein implicated in T-cell activation pathways. After initial synthesis of the peptide, the cytokine is proteolytically cleaved by proteases, thus separating the 23 residue signal sequence from the actual mature 232 residue 4-1BB Receptor sequence. The protein is expressed from the TNFRSF9 gene located at locus 1p36 on chromosome 1.

3604

Q07011

Cancer Immune Checkpoints

This particular immunoassay utilizes the quantitative technique of a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a sandwich format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human 4-1BBR cytokine while the user-added detection antibodies bind to epitopes on the captured target protein.