Human CD40 ELISA Kit, Lot 21AO-364 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P275
Product Type: ELISA Kit
Target: CD40
Short Description
The kit is designed for in vitro quantitative measurement of Human CD40 in Cell Lysate, Nuclear Extract.
Description
Human NF-κB p50 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NF-κB binding sequences to semi-quantitatively detect active NF-κB in lysates or nuclear extracts. Only available in North America.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
CD40
Reactivity
Human
Detection Method
Colorimetric
Method Type
DNA-Binding ELISA
Analytical Method
Semi-Quantitative
Sample Type
Cell Lysate, Nuclear Extract
Specificity
The olionucleotide/antibody pair provided in this kit recognizes human NF-κB p50 in whole lysates and nuclear extracts.
Components
96-well Strip Microplate pre-coated with DNA probes.
DNA Binding Buffer.
Positive Control Sample.
Specific Competitor DNA probe.
Non-specific Competitor DNA probe.
Assay Reagent.
DTT
Wash Buffer.
Primary Antibody.
HRP-conjugated Secondary Antibody.
TMB One-Step Substrate Reagent.
Stop Solution.
Material not included
Distilled or deionized water.
100 mL and 1 liter graduated cylinders.
Tubes to prepare sample dilutions Absorbent paper.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
Benchtop rocker or shaker.
Microplate reader capable of measuring absorbance at 450 nm
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Expiry Date
6 months
Note
Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Restrictions
For Research Use only
Alternative Name
p50
Synonyms
Bp50; CDW40; TNFRSF5; p50; AI326936; GP39; HIGM1; IGM; IMD3; T-BAM; TRAP; Tnfrsf5; TNFSF5; CD40 molecule; CD40 antigen; CD40; Cd40
Gene ID
958
UniProt
P25942
Pathways
NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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