Human CD40 Ligand (CD40LG) ELISA Kit, Lot 21AO-385 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human CD40 Ligand (CD40LG) in Cell Lysate, Serum, Plasma.

Human sCD40 Ligand ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human sCD40 Ligand concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme- Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.

ELISA

CD40 Ligand (CD40LG)

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Lysate, Serum, Plasma

The Human sCD40 Ligand ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human sCD40 Ligand proteins.

The Human sCD40 Ligand ELISA is capable of recognizing both recombinant and naturally produced Human sCD40 Ligand proteins. The antigens listed below were tested at 50 ng/mL and exhibited less than 1% cross reactivity. Murine: sCD40L The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. Human: 4-1BBL, BAFF, sFasL, FasR, IL-1α, sRANKL, sRANKR, sTNF-RI, sTNF-RII, TNFα, TNFβ, sTRAIL, sTRAIL-RI, sTRAIL-RII, TWEAK Murine: sRANKL, TNFα Rat: TNFα.

Soluble

Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips.
Protein Standard: Lyophilized (100 ng), Red container.
Biotinylated Detection Antibody: Lyophilized, Yellow container.
400x Streptavidin-HRP: 30 μL, Blue container.
Wash Buffer (10x): 50 mL, Clear containter.
Assay Diluent: 50 mL, Clear container.
Ready-to-Use Substrate: 12 mL, Brown container.
Stop Solution: 12 mL, Clear container.
Adhesive Plate Sealers: 4 Sheets.
Technical Manual 1 Manual.

Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm).
Micropipettes with capability of measuring volumes ranging from 1 μl to 1 mL.
Deionized or sterile water.
Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer.
Graph paper or computer software capable of generating or displaying logarithmic functions.
Absorbent paper or vacuum aspirator.
Test tubes or microfuge tubes capable of storing ≥1 mL.
Bench top centrifuge (optional).
Bench.
top vortex (optional).
Orbital shaker (optional).

Pre-coated

Addition of Known Standard and Unknown Sample to Immunoassay: The Human CD163 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD163 proteins.

1. Average the duplicate or triplicate readings for each standard, control and sample and subtract the average zero standard optical density.
2. Generate a standard curve by using Microsoft Excel or other computer software capable of establishing a 4- Parameter Logistic (4-PL) curve fit.

Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material.

Materials included in this kit should NOT be used past the expiration date on the kit label.
Reagents or substrates included in this kit should NOT be mixed or substituted with reagents or substrates from any other kits.

4 °C

Unopened Kits: Store at 4 °C for 6 months.
Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C.
Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

Unopened Kits: Store at 4 °C for 6 months.
Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C.
Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

For Research Use only

Cluster Of Differentiation 40 Ligand (CD40L)

CD154; cd40l; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39; hCD40L; CD40-L; Cd40l; Ly-62; Ly62; Tnfsf5; CD40 ligand; TNF superfamily member 5; CD40LG; cd40l; Cd40lg

Human sCD40 Ligand, also known as Tumor Necrosis Factor Ligand Superfamily Member 5, is a 149 amino acid cytokine protein encoded by the CD40LG gene located at locus Xq26 on the X chromosome. The soluble CD40 ligand is generated when the membrane form of the CD40 ligand undergoes proteolytic processing to remove the first 112 residues from the N-terminal region. The homotrimeric soluble CD40 ligand Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4, it is involved in immunoglobulin class switching.

959

P29965

Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints

This particular immunoassay utilizes the quantitative technique of a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a sandwich format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human sCD40 Ligand cytokine while the user-added detection antibodies bind to epitopes on the captured target protein.