Human CD40 & NF-κB Reporter Recombinant Cell Line, HEK293 (CAT#: IOC-LX138)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). This NF-κB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

Ideal for High Throughput Screening (HTS) to identify potential CD40 agonistic monoclonal antibodies and CD40-specific inhibitors.

Human

HEK293

CD40 & NF-κB Reporter

CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells and non-immune cells like endothelial cells and epithelial cells have been found to express CD40. A wide variety of carcinoma cells also over-express CD40. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells.