Human CD80 ELISA Kit, Lot 21AO-175 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P086
Product Type: ELISA Kit
Target: CD80
Short Description
The kit is designed for in vitro quantitative measurement of Human CD80 in Cell Culture Supernatant, Serum.
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human B7-1/CD80.
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells.
Target
CD80
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum
Specificity
Expression system for standard: NSO; Immunogen sequence: V35-N242
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 35-242
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl.
Sensitivity
<10pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD80 standard solution and each sample be measured in duplicate.
Assay Precision
Sample 1: n=16, Mean(ng/ml): 0.28, Standard deviation: 0.015, CV(%): 5.2
Sample 2: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.111, CV(%): 7.1
Sample 3: n=16, Mean(ng/ml): 2.31, Standard deviation: 0.113, CV(%): 4.9
Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.016, CV(%): 6.2
Sample 2: n=24, Mean(ng/ml): 1.72, Standard deviation: 0.138, CV(%): 8.0
Sample 3: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.134, CV(%): 5.4
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Synonyms
B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80 molecule; Cd80 molecule; CD80 antigen; CD80; Cd80
Background
The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1.The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.
Gene ID
941
UniProt
P33681
Pathways
TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Protocol
A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
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