Human CXCR4 ELISA Kit, Lot 21AO-110 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P021
Product Type: ELISA Kit
Target: CXCR4
Short Description
The kit is designed for in vitro quantitative measurement of Human CXCR4 in Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human CXC-chemokine receptor 4 (CXCR4) concentrations in serum, plasma, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
CXCR4
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human CXCR4. No significant cross-reactivity or interference between human CXCR4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human CXCR4 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
3.9 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Restrictions
For Research Use only
Alternative Name
Chemokine C-X-C-Motif Receptor 4 (CXCR4)
Synonyms
CXC-R4; CXCR-4; cd184; d2s201e; fb22; hm89; hsy3rr; lap3; lcr1; lestr; npy3r; npyr; npyrl; npyy3r; whim; xcxcr4; cxcr4; CD184; Cmkar4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r; D2S201E; FB22; HM89; HSY3RR; LAP3; LCR1; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; CXC-R4-B; CXCR-4-B; CXCR4; cb403; zgc:109863; C-X-C motif chemokine receptor 4; C-X-C chemokine receptor type 4; chemokine (C-X-C motif) receptor 4; C-X-C motif chemokine receptor 4 S homeolog; chemokine (C-X-C motif); receptor 4b; cxcr4; LOC100049444; CXCR4; Cxcr4; cxcr4.S; cxcr4b
Background
Abbreviation: CXCR4
Alias: CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, C-X-C chemokine receptor type 4|CD184 antigen|fusin|leukocyte-derived seven-transmembrane-domain receptor|lip
Gene ID
7852
UniProt
P61073
Pathways
Regulation of Cell Size, CXCR6-mediated Signaling Events
For Research Use Only | Not For Clinical Use
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