Human EGFR Custom rapid ELISA

CAT#: TM-HMM051
Product Type: Sandwich ELISA kit
Target: EGFR
Reactivity: Human
Applications: EGFR detection
Description
This product is a sandwich-based immunoassay with a simplified work flow for the rapid, quantitative measurement of human EGFR in only 3 hours.
Features
Compressed workflow with only one wash step and three incubation steps; 3 hours total processing time; Strip plates and additional reagents allow for use in multiple experiments; Quantitative protein detection; Establishes normal range; The best products for confirmation of antibody array data
Applications
EGFR detection
Target
EGFR
Reactivity
Human
Detection Method
Colorimetric
Method Type
Quantitative
Sample Type
Serum, plasma, cell culture supernatants, or lysate samples
Size
96-well microplate
Receptor Family
Epidermal growth factor family (EGF family)
Qualified With
Certificate of quality
Components
Streptavidin-coated 96-well strip microplate; Wash buffer; Stop solution; Assay diluent(s); Lyophilized standard; Biotinylated capture antibody; HRP conjugated detection antibody; TMB one-step substrate
Assay Time
3h
Plate
1, 2, or 5
Reagent Preparation
1.Distilled or deionized water 2.Precision pipettes to deliver 2 µL to 1 µL volumes 3.Adjustable 1-25 µL pipettes for reagent preparation 4.100 µL and 1 liter graduated cylinders 5.Tubes to prepare standard and sample dilutions 6.Absorbent paper 7.Microplate reader capable of measuring absorbance at 450nm 8.Log-log graph paper or computer and software for ELISA data analysis
Assay Procedure
1.Prepare all reagents, samples and standards as instructed in the manual; 2.Add 100 µL of standard or sample to each well; 3.Incubate 2.5 h at RT or O/N at 4°C; 4.Add 100 µL of prepared biotin antibody to each well; 5.Incubate 1 h at RT; 6.Add 100 µL of prepared Streptavidin solution to each well; 7.Incubate 45 min at RT; 8.Add 100 µL of TMB One-Step Substrate Reagent to each well; 9.Incubate 30 min at RT; 10.Add 50 µL of Stop Solution to each well; 11.Read at 450 nm immediately.
Target/Description
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
Accession Number
P00533
Storage
Stored for up to 6 months at 2° to 8°C from the date of shipment; Reconstituted standard can be stored at -80°C for up to 1 week; The kit can be used within one year if the whole kit is stored at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Synonyms
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Background
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
For Research Use Only | Not For Clinical Use
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