Human EGFR Sandwich ELISA Kit-4 pg/mL

CAT#: TM-HMM060
Product Type: Sandwich ELISA kit
Target: EGFR
Reactivity: Human
Applications: EGFR detection
Description
This product is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGFR in serum, plasma, and cell culture supernatants.
Applications
EGFR detection
Target
EGFR
Reactivity
Human
Detection Method
Colorimetric
Method Type
Quantitative
Sample Type
Cell culture supernatant, plasma, serum
Size
96-well microplate
Receptor Family
Epidermal growth factor family (EGF family)
Qualified With
Certificate of quality
Components
Wash buffer; HRP-streptavidin concentrate; Assay diluent B; Assay diluent A; Biotinylated anti-human EGFR; EGFR microplate; Recombinant human EGFR standard; Stop solution; TMB one-step substrate reagent
Sensitivity
4 pg/mL
Detection Range
2.74 pg/mL-2000 pg/mL
Assay Procedure
This assay employs an antibody specific for Human EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Target/Description
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
Storage
-20°C
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Synonyms
EGFR; EGF Receptor; ERBB; ERBB1; MENA; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1
Background
The epidermal growth factor receptor (EGFR) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands.
For Research Use Only | Not For Clinical Use
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